Ras Rabbit Monoclonal Antibody說(shuō)(shuō)明書(shū)(shū)

更新時(shí)(shí)間：2023-10-24 點(diǎn)(diǎn)擊次數：561

Ras Rabbit Monoclonal Antibody說(shuō)(shuō)明書(shū)(shū)

產(chǎn)(chǎn)品描述

About this Antibody
Name	Ras Rabbit Monoclonal Antibody
Category	Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype	IgG
Purification	Affinity purification
About the Immunogen
Immunogen	Recombinant protein
Gene ID	4893/3265/3845(Human); 16653/15461(Mouse); 24525/24605(Rat)
Synonyms	NS; NS3; CFC2; RALD; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B; c-Ki-ras2
Category	Cell Cycle; GPCR & G Protein
Background	Ras superfamily is a protein superfamily of small GTPases, which are all related, to a degree, to the Ras protein subfamily (the key human members of which are KRAS, NRAS, and HRAS). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway. GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway. The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein. This antibody is predicted to react with H-Ras, N-Ras and K-Ras.

注意事項

如果本抗體用于Western blot (WB)、免疫熒光(IF)、免疫細胞化學(xué)(xué)(ICC)等實(shí)(shí)驗，請注意回收使用過(guò)(guò)的稀釋抗體?；厥盞目貴w通常至少可以重復使用5-10次。稀釋后的抗體，包括已經(jīng)(jīng)使用過(guò)(guò)的稀釋抗體，請4℃保存。
回收后重復使用的抗體，使用方法同新鮮稀釋的抗體。如果在重復使用過(guò)(guò)程中發(fā)(fā)現抗體出現輕微混濁現象，可以10,000g離心1-3分鐘，取上清用于后續檢測。如果回收的抗體出現明顯的絮狀物或長(cháng)(cháng)霉長(cháng)(cháng)菌等情況，則可以考慮廢棄該抗體。
提供的Western一抗稀釋液也可以用于免疫熒光(IF)、免疫組化(IHC)、免疫細胞化學(xué)(xué)(ICC)等適當用途。如果希望獲得最佳的檢測效果，請考慮使用上述檢測專(zhuān)(zhuān)用的一抗稀釋液。
本產(chǎn)(chǎn)品僅限于專(zhuān)(zhuān)業(yè)(yè)人員的科學(xué)(xué)研究用，不得用于臨床診斷或治療，不得用于食品或藥品，不得存放于普通住宅內。
為了您的安全和健康，請穿實(shí)(shí)驗服并戴一次性手套操作。

運輸及保存方法

Ras Rabbit Monoclonal Antibody -20℃保存，Western一抗稀釋液-20℃或4℃保存，一年有效。Western一抗稀釋液優(yōu)(yōu)先推薦4℃保存，長(cháng)(cháng)期不使用可以考慮-20℃保存，但凍融可能會(huì )(huì )導致出現輕微的渾濁和少量不溶物。

貨號	品名	規格	品牌
78DA10003-50uL	Ras Rabbit Monoclonal Antibody	50uL	BIOHUB

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